diff --git a/libavformat/mxfenc.c b/libavformat/mxfenc.c
index d2e25a8824..8bc1a2807c 100644
--- a/libavformat/mxfenc.c
+++ b/libavformat/mxfenc.c
@@ -1760,7 +1760,7 @@ static void mxf_write_index_table_segment(AVFormatContext *s)
 
     // instance id
     mxf_write_local_tag(s, 16, 0x3C0A);
-    mxf_write_uuid(pb, IndexTableSegment, 0);
+    mxf_write_uuid(pb, IndexTableSegment, mxf->last_indexed_edit_unit);
 
     // index edit rate
     mxf_write_local_tag(s, 8, 0x3F0B);